新利官网 m.xl18.run|18luck新利官方网站

Skip to content Skip to search Skip to footer Ophthalmology & Visual Sciences Open Menu Back Close Menu Search for: Search Close Search Home About UsAbout Us Latest News and Announcements Faculty Directory History Becker Collection EducationEducation Residency ProgramResidency Program Meet the Residents What Distinguishes Us? Application Process Research OpportunitiesResearch Opportunities Ongoing and Completed Research Resident Call Schedule Subspecialty Rotations Living In St. Louis Frequently Asked Questions Clinical FellowshipsClinical Fellowships Meet the Fellows Corneal, External Disease, and Refractive Fellowship Glaucoma Fellowship Ocular Immunology/Uveitis Fellowship Ophthalmic Plastic &＃038; Reconstructive Surgery Fellowship Neuro-Ophthalmology Fellowship Pediatric Ophthalmology and Strabismus Fellowship Vitreoretinal Disease and Surgery Fellowship Optometry Residency Program ResearchResearch DOVS Labs ITVS Pathway T32 Vision Science Training Grant Vision Core GrantVision Core Grant Molecular Genetics Service Core Morphology &＃038; Imaging Core Vision Function Testing Core Graduate Course BIO5501 Student Research Opportunities LASIK Surgery Center Patient CarePatient Care Clinical Specialties Clinical Offices Clinical Trials Prepare for your visit Giving Contact UsContact Us Department Contacts AlumniAlumni Alumni Roster Alumni Videos PhotosPhotos Alumni Photos June 2012 Graduation Welcome Reception 2015 Hardesty Chair 5-31-12 Alumni Newsletters Association Dues EventsEvents Named Lectureship Series DOVS Grand Rounds Archives Open Search Morphology &＃038; Imaging Core The Morphology & Imaging module provides technical support in the processing, sectioning, staining, and morphological analysis of ocular cells and tissues at the light and electron microscopic level. Brief overview of histology services. Specialized tissue preparation Most users submit eye tissues for processing that have been chemically fixed by immersion in formaldehyde or similar fixatives.  However, ocular tissue is sometimes best preserved following transcardial perfusion with aldehyde-based fixatives.  This is a more time-consuming procedure but results in the rapid and comprehensive fixation of retina and other vascularized tissues. We can assist with this procedure.  Similarly, rapid freezing (in liquid pentane) followed by freeze substitution in methanol/acetic acid has recently been shown to optimally preserve ocular morphology and enhances staining with certain antibodies (see Sun et al.,2017 Mol Vis 21: 428-442).  We can assist with this procedure also. Embedding, sectioning, and staining. The lab performs tissue processing/embedding for routine paraffin samples, frozen sectioning, and glycol methacrylate. The lab offers routine H&E staining and a variety of special stains.  Immunohistochemistry with DAB or fluorescently-labeled secondary antibodies is available and we also offer in situ hybridization (RNAscope) and TUNEL labeling. Meet the Morphology & Imaging Core team Steven Bassnett, PhDGrace Nelson Lacy Distinguished Professor of Ophthalmology and Visual Science Email: [email protected] Bassnett Lab page Director of the Vision Center Core Director of the Imaging Core Guang-Yi (George) Ling Morphology core histology technician Phone: 3143623754 Email: [email protected] Located in McMillan Building, rooms 607/609 Yuefang Zhou, PhD Staff Scientist Phone: 314-362-1642 Email: [email protected] Below is a list of major equipment than can be used as part of this core Major Equipment Location (McMillan)  1 On-line Reservation 2 Training required Olympus FV1000 MPE confocal microscope 702B Yes Yes (Yuefang) Zeiss LSM800 Confocal microscope with Airyscan 702A Yes Yes (Yuefang) Olympus BX51 upright Fluorescence microscope 607 Yes Yes (Yuefang) Olympus BX40 double-headed microscope 607 No No Leica Fluorescence Macroscope 622 No No Leica LMD6000 laser microdissection system 607 Yes Yes (Yuefang) Bioptigen small animal OCT 606 Yes Yes (Guang-Yi) Sakura Tissue-Tek VIP tissue processor 609 n/a n/a Shandon Cryotome E cryostat 609 Yes Yes (Guang-Yi) Tissue-Tek Paraffin embedding center 609 n/a n/a Leica RM2255 microtome 609 n/a n/a Leica Ultracut microtome 607 n/a n/a In situ hybridization oven 609 Yes Yes (Guang-Yi) Image Processing computer with Metamorph software 603 Yes No To reserve equipment 1 On-line reservation is available through Facility Online Manager (FOM). The FOM website address is www.instrumentschedule.com.  Our equipment is listed under WUSTL-VISION. You will need to create a user account in order to access the calendar and reserve time on the instrumentation.   2 Only individuals trained in the use of this instrumentation will be allowed to reserve time through the FOM website. For detailed instructions, contact Yuefang Zhou ([email protected]). Fee Schedule (FY24)  Confocal microscopy To offset the cost of the maintenance contracts on the confocal microscopes there is an annual user fee of $2,300 per lab.  This allows unlimited access to the instruments. For occasional users, an hourly fee of $50 is charged.   Immunomorphology service request form Histology pricing ServiceUnit Price ($)Tissue processing/embedding – paraffin (per block)3.97Tissue processing – frozen (per block) 3.36Slides – paraffin – unstained (per slide) 0.96Slides – frozen – unstained (per slide) 0.61Histochemical stain (H&E, Toluidine blue, etc) (per slide)2.95H&E stain only (per slide)1.99Immunocytochemistry (with user-provided 1o antibody) per slide 3.91TUNEL labeling (to detect apoptotic cells) per slide18.29 Research Graduate Course BIO5501 ITVS Pathway T32 Vision Science Training Grant DOVS Labs Vision Core Researchers Student Research Opportunities Vision Core Grant Biostatistics and Bioinformatics Core Molecular Genetics Service Core Morphology &＃038; Imaging Core Vision Function Testing Core STAY CONNECTED Join our newsletter list! Subscribe Now John F. Hardesty, MD, Department of Ophthalmology & Visual SciencesWashington University School of MedicineContact Us Facebook Instagram Twitter YouTube AFFILIATE INSTITUTIONS LINKS Faculty Job Openings Staff Job Openings Employee Portal (login required) Join our e-Newsletter mailing list ©2024 Washington University in St. Louis